One of the most astonishing and useful developments in the worlds of biology and biophysics in recent years has been the rapid advent of techniques such as Photoactivated Localization Microscopy (PALM) and Fluorescence Resonance Energy Transfer (FRET), which allow researchers to construct images with molecular-scale resolution, far finer than Abbe's classical diffraction limit allows. The field--collectively termed Super-Resolution Microscopy--is illuminating biological structures that are the fundamental scaffolding of life, the framework of disease, and the stepping-stones to cures.
The prestigious journal Nature has produced an instructive online video which provides a concise overview of this field:
More articles on nanopositioning applications in microscopy and imaging
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